Design for Manufacture and Assembly (DfMA) has emerged as a promising approach to improving productivity in the construction industry. In the most recent studies, the most widespread adoption of DfMA is expected in off-site construction (OSC), so there is a misconception that DfMA is only for OSC, whereas not all projects are suitable for OSC or even entirely for OSC, but can still benefit from DfMA’s design principles. In this context, “on-site construction” refers to work carried out on site as part of an OSC project, as well as to specific projects carried out on the site itself that are not considered traditional construction. Given that the adoption of DfMA as a systematic procedure is set to become widespread in the construction sector, it is essential to identify the challenges associated with its implementation at the various stages of a construction process. This article aims to identify, verify and analyze the challenges of DfMA, focusing on on-site construction. The study used a mixed-methods approach, including an in-depth literature review and interviews with industry experts. The information gathered from the literature review and expert contributions was analyzed and synthesized using NVivo 14 pro and prioritized using mean score (MS) analysis and the weighting function.