Design for Manufacturing and Assembly (DfMA) has emerged as a promising approach to enhance productivity in the construction industry. In the most recent studies, the most widespread DfMA adoption is foreseen in off-site construction (OSC), so there is a misconception that DfMA serves OSC only, while not all projects are suitable for OSC or even fully OSC, but can still benefit from DfMA design principles. In this context, « on-site construction » refers to work completed on-site as part of an OSC project as well as specific projects that are carried out at the actual location that are not considered traditional constructions. Since the adoption of DfMA as a systematic procedure is anticipated to be widespread within the construction sector, it is crucial to identify Challenges associated with its implementation in the different stages of a construction process. This article aims to identify, verify, and analyze the DfMA challenges with a focus on the on-site constructions. The study utilized a mixed-method approach, comprising an extensive review of the literature and industry expert interviews. The information gathered from the literature review and expert inputs was analyzed and synthesized using NVivo 14 pro and prioritized using the mean score (MS) analysis and weighting function.